Optimizing Process Parameters for Efficient Supercritical CO₂ Extraction of Seed Oils: A Review
Keywords:
Supercritical CO₂ extraction, Seed oils, Process optimization, Response surface methodology, Modeling, Green technology, Bioactive lipidsAbstract
Supercritical carbon dioxide (SC-CO₂) extraction has emerged as one of the most promising green technologies for recovering high-value seed oils enriched with bioactive lipids, antioxidants, tocopherols, and essential fatty acids. As a tunable solvent whose density and solvating power can be precisely controlled through pressure and temperature, SC-CO₂ provides a clean, efficient, and selective alternative to conventional solvent-based extraction methods. This review comprehensively examines the influence of key process parameters—pressure, temperature, particle size, CO₂ flow rate, extraction time, and co-solvent ratio—on oil yield, composition, and functional quality. Across numerous studies, optimal operating conditions typically fall within 20–35 MPa and 40–70 °C, where the balance between solvent density and volatility promotes maximal solubility of triacylglycerols and unsaponifiable compounds. Particle sizes between 0.5 and 0.7 mm consistently enhance extraction efficiency by reducing internal diffusion resistance while maintaining bed permeability and minimizing channeling. The review also highlights the strategic use of 5–10% ethanol as a co-solvent to increase solvent polarity and facilitate the recovery of moderately polar constituents such as phenolics, sterols, and tocopherols. These enhancements are strongly supported by statistical optimization methods, particularly Response Surface Methodology (RSM), which effectively captures interaction effects among variables. Moreover, Artificial Neural Networks (ANN) and Genetic Algorithms (GA) provide powerful nonlinear predictive capabilities, enabling more robust optimization for maximizing yield and tailoring compositional outcomes. To complement experimental findings, empirical and semi-empirical solubility models—including the Chrastil equation, Sovová’s mass-transfer model, and the Peng–Robinson equation of state—are widely applied to describe phase behavior, predict solute solubility, and simulate extraction kinetics under varying conditions. The synthesis of current literature confirms that SC-CO₂ extraction offers significant advantages in terms of product purity, environmental safety, and operational selectivity. By eliminating organic solvent residues and supporting energy-efficient processing, SC-CO₂ aligns closely with the principles of green chemistry and the broader movement toward sustainable biorefinery technologies. This review underscores the important role of parameter optimization, modeling tools, and process integration in advancing SC-CO₂ extraction as a scalable and environmentally responsible method for high-quality seed oil production.
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[1] F. Fahrullah, D. Kisworo, and A. Noersidiq, "Synthesis of Whey-Chia Seed Edible Film containing Cinnamon Essential Oil as Biodegradable Food Packaging Material," Journal of Food Quality and Hazards Control, Article vol. 12, no. 1, pp. 11-26, 2025, doi: 10.18502/jfqhc.12.1.18363.
[2] S. Onder, D. Onder, Ü. Erdoğan, S. Waleed Khalid Khalid, and M. Tonguç, "Composition and antioxidant activity of seed oil of date palm (Phoenix dactylifera L.) cultivars from Iraq," Genetic Resources and Crop Evolution, Article vol. 72, no. 4, pp. 3939-3952, 2025, doi: 10.1007/s10722-024-02319-2.
[3] M. A. Al-Hussain et al., "Evaluation of the fatty acid profiles, antioxidant activities, and total phenol and vitamin E contents of three types of Saudi date seed oils," Food Research, Article vol. 9, no. 1, pp. 87-92, 2025, doi: 10.26656/fr.2017.9(1).080.
[4] A. McGurrin et al., "Antimicrobial Activities of Polysaccharide-Rich Extracts from the Irish Seaweed Alaria esculenta, Generated Using Green and Conventional Extraction Technologies, Against Foodborne Pathogens," Marine Drugs, Article vol. 23, no. 1, 2025, Art no. 46, doi: 10.3390/md23010046.
[5] F. Dias De Ávila et al., "Bio-Oil Production from Fish Processing Waste Residues Using Oleaginous Rhodotorula sp. R1 After Conventional Oil Extraction," (in English), Bioenergy Research, Article vol. 17, no. 3, pp. 1885-1894, 2024, doi: 10.1007/s12155-024-10749-0.
[6] S. Bayrak, M. Sökmen, E. Aytaç, and A. Sökmen, "Conventional and supercritical fluid extraction (SFE) of colchicine from Colchicum speciosum," Industrial Crops and Products, Article vol. 128, pp. 80-84, 2019, doi: 10.1016/j.indcrop.2018.10.060.
[7] Y. Ozogul, Y. Ucar, F. Takadaş, M. Durmus, A. R. Köşker, and A. Polat, "Comparision of Green and Conventional Extraction Methods on Lipid Yield and Fatty Acid Profiles of Fish Species," (in English), European Journal of Lipid Science and Technology, Article vol. 120, no. 12, 2018, Art no. 1800107, doi: 10.1002/ejlt.201800107.
[8] S. Minaei, A. Saebi, S. MostasharShahidi, A. Mahdavian, M. T. Ebadi, and M. Markom, "Thermodynamic optimization of a staged supercritical CO₂ system for high-purity bioactive separation from coriander seeds," Journal of Supercritical Fluids, Article vol. 227, 2026, Art no. 106756, doi: 10.1016/j.supflu.2025.106756.
[9] M. K. M. Lane, E. B. Gilcher, M. M. Ahrens-Víquez, R. S. Pontious, N. E. Wyrtzen, and J. B. Zimmerman, "Elucidating supercritical fluid extraction of fucoxanthin from algae to enable the integrated biorefinery," Bioresource Technology, Article vol. 406, 2024, Art no. 131036, doi: 10.1016/j.biortech.2024.131036.
[10] S. N. S. Abdul Rahman, Y. H. Chai, and M. K. Lam, "Taguchi approach for assessing supercritical CO2 (sCO2) fluid extraction of polyhydroxyalkanoate (PHA) from Chlorella Vulgaris sp. microalgae," Journal of Environmental Management, Article vol. 355, 2024, Art no. 120447, doi: 10.1016/j.jenvman.2024.120447.
[11] Y. R. Shin, V. C. Roy, J. S. Park, W. Zhang, and B. S. Chun, "Consecutive extraction of neutral and polar lipids from skipjack tuna (Katsuwonus pelamis) byproducts using supercritical carbon dioxide," (in English), Journal of Supercritical Fluids, Article vol. 206, 2024, Art no. 106175, doi: 10.1016/j.supflu.2024.106175.
[12] A. Balkrishna, P. Nain, M. Joshi, L. Khandrika, and A. Varshney, "Supercritical fluid extract of putranjiva roxburghii wall. Seeds mitigates fertility impairment in a zebrash model," (in English), Molecules, Article vol. 26, no. 4, 2021, Art no. 1020, doi: 10.3390/molecules26041020.
[13] R. Melgosa et al., "Supercritical CO2 and subcritical water technologies for the production of bioactive extracts from sardine (Sardina pilchardus) waste," (in English), Journal of Supercritical Fluids, Article vol. 164, 2020, Art no. 104943, doi: 10.1016/j.supflu.2020.104943.
[14] V. C. Roy, A. T. Getachew, Y. J. Cho, J. S. Park, and B. S. Chun, "Recovery and bio-potentialities of astaxanthin-rich oil from shrimp ((Peneanus monodon) waste and mackerel (Scomberomous niphonius) skin using concurrent supercritical CO2 extraction," (in English), Journal of Supercritical Fluids, Article vol. 159, 2020, Art no. 104773, doi: 10.1016/j.supflu.2020.104773.
[15] N. R. Putra, S. Suharmiati, R. Ismayanti, I. Irianto, and B. Airlangga, "Green extraction of chamomile bioactives: techniques, health benefits, and wellness applications–a comprehensive review," Journal of Essential Oil Research, pp. 1-16, 2025.
[16] B. Suryawanshi and B. Mohanty, "Modeling and optimization: Supercritical CO2 extraction of Pongamia pinnata (L.) seed oil," Journal of Environmental Chemical Engineering, Article vol. 6, no. 2, pp. 2660-2673, 2018, doi: 10.1016/j.jece.2018.04.014.
[17] T. O. Kehinde, O. A. Bhadmus, and J. Olufelo, "Influence of plant extracts, storage containers and storage duration on the physiological quality of watermelon (Citrullus lanatus (Thunb.) Mansf.) seeds stored under ambient conditions," Acta Agriculturae Slovenica, Article vol. 116, no. 2, pp. 229-236, 2021, doi: 10.14720/aas.2020.116.2.1440.
[18] N. R. Putra et al., "Supercritical Carbon Dioxide Extraction of Citronella Oil Review: Process Optimization, Product Quality, and Applications," Pertanika Journal of Science and Technology, 2024.
[19] N. F. M. Idrus et al., "Mini review: Application of supercritical carbon dioxide in extraction of propolis extract," J. Malays. J. Fundam. Appl. Sci, vol. 14, pp. 387-396, 2018.
[20] N. R. Putra et al., "Influence of particle size in supercritical carbon dioxide extraction of roselle (Hibiscus sabdariffa) on bioactive compound recovery, extraction rate, diffusivity, and solubility," Scientific Reports, vol. 13, no. 1, p. 10871, 2023.
[21] M. Alboofetileh, S. Jeddi, and M. Abdollahi, "Sequential recovery of alginate from fucoidan extraction by-products of Nizamuddinia zanardinii seaweed using green extraction methods," Ultrasonics Sonochemistry, Article vol. 117, 2025, Art no. 107343, doi: 10.1016/j.ultsonch.2025.107343.
[22] I. Irianto, S. Suharmiati, A. S. Zaini, M. A. Ahmad Zaini, B. Airlanngga, and N. R. Putra, "Sustainable innovations in garlic extraction: A comprehensive review and bibliometric analysis of green extraction methods," Green Processing and Synthesis, vol. 14, no. 1, p. 20240201, 2025.
[23] Q. Li, N. R. Putra, D. N. Rizkiyah, A. H. Abdul Aziz, I. Irianto, and L. Qomariyah, "Orange pomace and peel extraction processes towards sustainable utilization: A short review," Molecules, vol. 28, no. 8, p. 3550, 2023.
[24] N. Marčac Duraković et al., "Recovery of Fennel Non-Polar Bioactives via Supercritical Carbon Dioxide Extraction," Processes, Article vol. 12, no. 8, 2024, Art no. 1764, doi: 10.3390/pr12081764.
[25] S. Pothinam, T. Siriwoharn, and W. Jirarattanarangsri, "Optimization of perilla seed oil extraction using supercritical CO2," Quality Assurance and Safety of Crops and Foods, Article vol. 17, no. 1, pp. 14-29, 2025, doi: 10.15586/qas.v17i1.1509.
[26] O. Dhara, T. Azmeera, A. Eanti, and P. P. Chakrabarti, "Garden cress oil as a vegan source of PUFA: Achieving through optimized supercritical carbon dioxide extraction," (in English), Innovative Food Science and Emerging Technologies, Article vol. 84, 2023, Art no. 103283, doi: 10.1016/j.ifset.2023.103283.
[27] B. Shrirame et al., "Supercritical CO2extraction of caraway (Carum carvi L.) seed: Optimization and parametric interaction studies using design of experiments," Journal of CO2 Utilization, Article vol. 65, 2022, Art no. 102195, doi: 10.1016/j.jcou.2022.102195.
[28] K. Buranachokpaisan, R. Muangrat, and Y. Chalermchat, "Supercritical CO2 extraction of residual oil from pressed sesame seed cake: Optimization and its physicochemical properties," Journal of Food Processing and Preservation, Article vol. 45, no. 9, 2021, Art no. e15722, doi: 10.1111/jfpp.15722.
[29] M. Guta, H. Tan, and Y. Zhao, "Response surface optimization for supercritical carbon dioxide extraction of Korarima (Aframomum corrorima) seed oil and its antibacterial activity evaluation," Journal of Supercritical Fluids, Article vol. 215, 2025, Art no. 106411, doi: 10.1016/j.supflu.2024.106411.
[30] F. Reinoso et al., "Enzymatic Interesterification of Cold-Pressed Maqui (Aristotelia chilensis (Mol.) Stuntz) Seed Oil and Belly Oil from Rainbow Trout (Oncorhynchus mykiss) Through Supercritical CO2," Marine Drugs, Article vol. 22, no. 12, 2024, Art no. 547, doi: 10.3390/md22120547.
[31] S. Milovanović et al., "A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds," Food. Bioprocess Technol., Article vol. 16, no. 8, pp. 1806-1821, 2023, doi: 10.1007/s11947-023-03038-9.
[32] P. Pao-La-Or, B. Marungsri, K. Posridee, R. Oonsivilai, and A. Oonsivilai, "Supercritical CO2 Extraction of Seed Oil from Psophocarpus tetragonolobus (L.) DC.: Optimization of Operating Conditions through Response Surface Methodology and Probabilistic Neural Network," Processes, Article vol. 11, no. 7, 2023, Art no. 1949, doi: 10.3390/pr11071949.
[33] B. Mazurek, M. Wójciak, D. Kostrzewa, and M. Kondracka, "Modeling and Optimization of the Isolation of Blackcurrant and Black Cumin Seeds Oils Using Supercritical Fluid Extraction," Molecules, Article vol. 27, no. 24, 2022, Art no. 8921, doi: 10.3390/molecules27248921.
[34] I. Lukić, J. Pajnik, V. Tadić, and S. Milovanović, "Supercritical CO2-assisted processes for development of added-value materials: Optimization of starch aerogels preparation and hemp seed extracts impregnation," Journal of CO2 Utilization, Article vol. 61, 2022, Art no. 102036, doi: 10.1016/j.jcou.2022.102036.
[35] G. Gawron, W. Krzyczkowski, R. Łyżeń, L. Kadziński, and B. Banecki, "Influence of supercritical carbon dioxide extraction conditions on extraction yield and composition of nigella sativa l. Seed oil—modelling, optimization and extraction kinetics regarding fatty acid and thymoquinone content," Molecules, Article vol. 26, no. 21, 2021, Art no. 6419, doi: 10.3390/molecules26216419.
[36] G. Ferrentino, S. Giampiccolo, K. Morozova, N. Haman, S. Spilimbergo, and M. Scampicchio, "Supercritical fluid extraction of oils from apple seeds: Process optimization, chemical characterization and comparison with a conventional solvent extraction," Innovative Food Science and Emerging Technologies, Article vol. 64, 2020, Art no. 102428, doi: 10.1016/j.ifset.2020.102428.
[37] W. L. Peng, H. Mohd-Nasir, S. H. M. Mohd.-Setapar, A. Ahmad, and D. Lokhat, "Optimization of process variables using response surface methodology for tocopherol extraction from Roselle seed oil by supercritical carbon dioxide," Industrial Crops and Products, Article vol. 143, 2020, Art no. 111886, doi: 10.1016/j.indcrop.2019.111886.
[38] Z. J. Luan, P. P. Li, D. Li, X. P. Meng, and J. Sun, "Optimization of supercritical-CO2 extraction of Iris lactea seed oil: Component analysis and antioxidant activity of the oil," Industrial Crops and Products, Article vol. 152, 2020, Art no. 112553, doi: 10.1016/j.indcrop.2020.112553.
[39] M. Chouaibi, K. Rigane, and G. Ferrari, "Extraction of Citrullus colocynthis L. seed oil by supercritical carbon dioxide process using response surface methodology (RSM) and artificial neural network (ANN) approaches," Industrial Crops and Products, Article vol. 158, 2020, Art no. 113002, doi: 10.1016/j.indcrop.2020.113002.
[40] n. Priyanka and S. Khanam, "Supercritical CO2 extraction of carrot seed oil: screening, optimization and economic analysis," International Journal of Environmental Science and Technology, Article vol. 17, no. 4, pp. 2311-2324, 2020, doi: 10.1007/s13762-019-02497-y.
[41] B. Suryawanshi and B. Mohanty, "Modeling and optimization of process parameters for supercritical CO 2 extraction of Argemone mexicana (L.) seed oil," Chemical Engineering Communications, Article vol. 206, no. 8, pp. 1087-1106, 2019, doi: 10.1080/00986445.2018.1547712.
[42] B. Pavlić et al., "Supercritical fluid extraction of raspberry seed oil: Experiments and modelling," Journal of Supercritical Fluids, Article vol. 157, 2020, Art no. 104687, doi: 10.1016/j.supflu.2019.104687.
[43] V. Devi and S. Khanam, "Study of ω-6 linoleic and ω-3 α-linolenic acids of hemp (Cannabis sativa) seed oil extracted by supercritical CO 2 extraction: CCD optimization," Journal of Environmental Chemical Engineering, Article vol. 7, no. 1, pp. 1-10, 2019, Art no. 102818, doi: 10.1016/j.jece.2018.102818.
[44] S. Bilgiç-Keleş, N. Sahin-Yesilcubuk, A. Barla-Demirkoz, and M. Karakaş, "Response surface optimization and modelling for supercritical carbon dioxide extraction of Echium vulgare seed oil," Journal of Supercritical Fluids, Article vol. 143, pp. 365-369, 2019, doi: 10.1016/j.supflu.2018.09.008.
[45] A. Abdullah, S. S. A. Gani, N. F. M. Mokhtar, T. Y. Y. Yap, Z. A. Zaibunnisa, and S. Mustafa, "Supercritical carbon dioxide extraction of red pitaya (Hylocereus polyrhizus) seeds: Response surface optimization, fatty acid composition and physicochemical properties," Malays. Appl. Biol., Article vol. 47, no. 2, pp. 39-46, 2018. [Online]. Available: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047667792&partnerID=40&md5=40231ae9b98e0e0c173ce629f321d515.
[46] G. Sodeifian, S. A. Ali Sajadian, and B. Honarvar, "Mathematical modelling for extraction of oil from Dracocephalum kotschyi seeds in supercritical carbon dioxide," Natural Product Research, Article vol. 32, no. 7, pp. 795-803, 2018, doi: 10.1080/14786419.2017.1361954.
[47] G. Wejnerowska and A. Ciaciuch, "Optimisation of oil extraction from Quinoa seeds with supercritical carbon dioxide with co-solvents," Czech J. Food Sci., Article vol. 36, no. 1, pp. 81-87, 2018, doi: 10.17221/122/2017-CJFS.
[48] J. Ndayishimiye and B. S. Chun, "Optimization of carotenoids and antioxidant activity of oils obtained from a co-extraction of citrus (Yuzu ichandrin) by-products using supercritical carbon dioxide," Biomass and Bioenergy, Article vol. 106, pp. 1-7, 2017, doi: 10.1016/j.biombioe.2017.08.014.
[49] D. Panadare, G. Dialani, and V. Rathod, "Extraction of volatile and non-volatile components from custard apple seed powder using supercritical CO2 extraction system and its inventory analysis," Process Biochemistry, Article vol. 100, pp. 224-230, 2021, doi: 10.1016/j.procbio.2020.09.030.
[50] A. Rai, B. Mohanty, and R. Bhargava, "Experimental Modeling and Simulation of Supercritical Fluid Extraction of Moringa oleifera Seed Oil by Carbon Dioxide," Chemical Engineering Communications, Article vol. 204, no. 8, pp. 957-964, 2017, doi: 10.1080/00986445.2017.1328415.
[51] J. P. Prakash Maran and B. Priya, "Supercritical fluid extraction of oil from muskmelon (Cucumis melo) seeds," Journal of the Taiwan Institute of Chemical Engineers, Article vol. 47, pp. 71-78, 2015, doi: 10.1016/j.jtice.2014.10.007.
[52] J. M. Mohammed Danlami, M. A. A. Zaini, A. Arsad, and M. A. C. Che Yunus, "A parametric investigation of castor oil (Ricinus comminis L) extraction using supercritical carbon dioxide via response surface optimization," Journal of the Taiwan Institute of Chemical Engineers, Article vol. 53, pp. 32-39, 2015, doi: 10.1016/j.jtice.2015.02.033.
[53] C. Wang, Z. Duan, L. Fan, and J. Li, "Supercritical CO2 fluid extraction of elaeagnus mollis diels seed oil and its antioxidant ability," Molecules, Article vol. 24, no. 5, 2019, Art no. 911, doi: 10.3390/molecules24050911.
[54] X. Y. Liu, H. Ou, H. Gregersen, and J. Zuo, "Supercritical carbon dioxide extraction of Cosmos sulphureus seed oil with ultrasound assistance," Journal of CO2 Utilization, Article vol. 70, 2023, Art no. 102429, doi: 10.1016/j.jcou.2023.102429.
[55] F. Gashi, C. Turner, A. Mustafa, and F. Nermark, "A sustainable approach to extracting baobab oil: neat supercritical CO2 optimization," RSC Advances, Article vol. 15, no. 27, pp. 21803-21810, 2025, doi: 10.1039/d5ra02490k.
[56] J. Maitusong, A. Aimila, J. Zhang, B. Bakri, M. Maiwulanjiang, and H. A. Aisa, "Process optimization for the supercritical carbon dioxide extraction of Foeniculum vulgare Mill. seeds aromatic extract with respect to yield and trans-anethole contents using Box-Behnken design," Flavour and Fragrance Journal, Article vol. 36, no. 2, pp. 280-291, 2021, doi: 10.1002/ffj.3643.
[57] B. Suryawanshi and B. Mohanty, "Application of an artificial neural network model for the supercritical fluid extraction of seed oil from Argemone mexicana (L.) seeds," Industrial Crops and Products, Article vol. 123, pp. 64-74, 2018, doi: 10.1016/j.indcrop.2018.06.057.
[58] Q. Sun, J. Shi, M. Scanlon, S. J. Xue, and J. Lu, "Optimization of supercritical-CO2 process for extraction of tocopherol-rich oil from canola seeds," LWT, Article vol. 145, 2021, Art no. 111435, doi: 10.1016/j.lwt.2021.111435.
[59] A. Rai, B. Mohanty, and R. Bhargava, "Fitting of broken and intact cell model to supercritical fluid extraction (SFE) of sunflower oil," Innovative Food Science and Emerging Technologies, Article vol. 38, pp. 32-40, 2016, doi: 10.1016/j.ifset.2016.08.019.
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